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Editorial

hTRIR的功能洞察

卷 24, 期 12, 2024

发表于: 20 October, 2023

页: [1445 - 1449] 页: 5

弟呕挨: 10.2174/0115665240260310231016112946

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摘要

未表征的C19orf43被发现与hTR成熟有关。我们之前的工作表明，C19orf43可以切割不同类型的RNA，但不能切割DNA。然后我们将其命名为htr相互作用rna (hTRIR) （Uniprot: Q9BQ61）。hTRIR在很宽的温度和pH范围内工作，不需要任何二价阳离子。hTRIR在所有四个核苷酸位点切割RNA，但优先在嘌呤位点切割。此外，hTRIR酶切了甲基化小RNA的两端，这表明它是一种假定的核糖核酸酶。后来，我们设计了更多的核苷酸来甲基化小RNA，以确定它是外核酸酶和/或核糖核酸内酶。与RNase A不同，hTRIR可以消化甲基化RNA寡核苷酸5R5的两端，这表明它可能是一种核糖核酸内酶。

关键词: 核糖核酸内切酶，hTRIR，甲基化RNA，核糖核酸内切酶，RNA酶，RNA消化。

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