Synthesis of the Peptide Ac-Wahx-KTTKS and Evaluation of the Ability to Induce In Vitro Collagen Synthesis

Title:Synthesis of the Peptide Ac-Wahx-KTTKS and Evaluation of the Ability to Induce In Vitro Collagen Synthesis

Volume: 23 Issue: 6

Author(s): Danila A.S. Guglielmi, Ariane M. Martinelli, Nathália C. Rissi, Eduardo M. Cilli, Christiane P. Soares and Leila A. Chiavacci

Affiliation:

Keywords: Ac-WAhx-KTTKS, antiaging, collagen biosynthesis, cytotoxicity, peptide synthesis, skin functions.

Abstract: In this work, we report the synthesis of a peptide analogue of the KTTKS, termed Ac- Wahx-KTTKS and evaluate its cytotoxicity and role in biosynthesis of collagen for future application in skin aging. The peptide was obtained with purity higher than 97.5%. In the cytotoxicity assay, we observed non-toxic effects for Ac-WAhx-KTTKS at concentrations below 600 µM for HaCaT and 500 µM for HepG2 cells, respectively. After 24 and 48 h it was possible to observe significant changes in collagen synthesis in the groups treated with various concentrations of the peptide. In conclusion, the Ac-Wahx- KTTKS peptide increased collagen synthesis in fibroblasts by 80% and it is a promising candidate for improving skin aging.

Cite this article as:

A.S. Guglielmi Danila, M. Martinelli Ariane, C. Rissi Nathália, M. Cilli Eduardo, P. Soares Christiane and A. Chiavacci Leila, Synthesis of the Peptide Ac-Wahx-KTTKS and Evaluation of the Ability to Induce In Vitro Collagen Synthesis, Protein & Peptide Letters 2016; 23 (6) . https://dx.doi.org/10.2174/0929866523666160331144315