Characterization of Pearl Millet Prolamins

Lucilia   Helena   Marcellino; Carlos   Bloch   Junior; Eugen   Silvano   Gander

doi:10.2174/0929866023408724

Abstract

We report the physical-chemical characterization of the major alcohol-soluble proteins present in seeds of pearl millet (Pennisetum glaucum) by SDS-PAGE, bidimensional gel electrophoresis, MALDI-TOF / MS and RP-HPLC. We demonstrate the presence of three major prolamins, called A-, B- and C-pennisetin with mass values around 27, 22 and 12 kDa, respectively. We present partial amino acid sequences of these major proteins, which should allow the posterior isolation of the respective genes.

Keywords: pearl millet prolamin, pennisetum galaucum, seed storage protein, prolamin, pennisetin

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Protein & Peptide Letters

Title: Characterization of Pearl Millet Prolamins

Volume: 9 Issue: 3

Author(s): Lucilia Helena Marcellino, Carlos Bloch Junior and Eugen Silvano Gander

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Keywords: pearl millet prolamin, pennisetum galaucum, seed storage protein, prolamin, pennisetin

Abstract: We report the physical-chemical characterization of the major alcohol-soluble proteins present in seeds of pearl millet (Pennisetum glaucum) by SDS-PAGE, bidimensional gel electrophoresis, MALDI-TOF / MS and RP-HPLC. We demonstrate the presence of three major prolamins, called A-, B- and C-pennisetin with mass values around 27, 22 and 12 kDa, respectively. We present partial amino acid sequences of these major proteins, which should allow the posterior isolation of the respective genes.

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Cite this article as:

Marcellino Helena Lucilia, Junior Bloch Carlos and Gander Silvano Eugen, Characterization of Pearl Millet Prolamins, Protein & Peptide Letters 2002; 9 (3) . https://dx.doi.org/10.2174/0929866023408724

DOI https://dx.doi.org/10.2174/0929866023408724	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305

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