Affinity of Chicken Cystatin for S- (Carbamoylmethyl)-Papain, Measured by Fluorescence at Acidic ph

Alfonso      Arroyo-Reyna; Ezequiel      Gutierrez-Gomez

doi:10.2174/0929866023408797

Abstract

We have characterized the interaction between S-(carbamoylmethyl)-papain and chicken cystatin by means of fluorescence titration at acidic pH. The calculated binding constant was 32 x 10 6, which is less than the tight affinity at pH 7.4 (∼7 x 10 10). Results have been discussed in terms of the burial of Trp-104 of cystatin and the possible role of the charge of the carbamoylmethyl moiety.

Keywords: carbamoylmethyl-papain, chicken cystatin

« Previous Next »

Rights & Permissions Print Cite

Protein & Peptide Letters

Title: Affinity of Chicken Cystatin for S- (Carbamoylmethyl)-Papain, Measured by Fluorescence at Acidic ph

Volume: 9 Issue: 3

Author(s): Alfonso Arroyo-Reyna and Ezequiel Gutierrez-Gomez

Affiliation:

Keywords: carbamoylmethyl-papain, chicken cystatin

Abstract: We have characterized the interaction between S-(carbamoylmethyl)-papain and chicken cystatin by means of fluorescence titration at acidic pH. The calculated binding constant was 32 x 10 6, which is less than the tight affinity at pH 7.4 (∼7 x 10 10). Results have been discussed in terms of the burial of Trp-104 of cystatin and the possible role of the charge of the carbamoylmethyl moiety.

Export Options

Export File:

RIS (for EndNote, Reference Manager, ProCite)

BibTeX

Text

Content:

Citation Only

Citation and Abstract

About this article

Cite this article as:

Arroyo-Reyna Alfonso and Gutierrez-Gomez Ezequiel, Affinity of Chicken Cystatin for S- (Carbamoylmethyl)-Papain, Measured by Fluorescence at Acidic ph, Protein & Peptide Letters 2002; 9 (3) . https://dx.doi.org/10.2174/0929866023408797

DOI https://dx.doi.org/10.2174/0929866023408797	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305

About this journal

Call for Papers in Thematic Issues

Submission closes on : 31 December, 2025

Therapeutic Proteins and Peptides of Plant Origin

Plants are still the major repository of biologically active substances. In the last two decades, however, natural peptides and proteins of plant origin have gained increasing attention due to their pharmacological activities over a variety of human illnesses, including those mediated by infections and parasitosis and those involving different cellular ...read more

Guest Editor(s): Dr. Márcio Viana Ramos