Fluorescence Instrumental and Techniques

Abstract

The use of fluorescence microscopy requires knowledge of the elements making up the fluorescence microscope. In this chapter the basic elements combining to make a fluorescence microscope will be introduced and its role explained. Afterwards an introduction to the most common fluorescence microscopic techniques will be made followed by more advanced and developed approaches. These will include total-internal reflection and time-resolved microscopies, to name a few. The chapter will end with a brief introduction to flow cytometry, a highly related fluorescence technique in the biomedical field.

Keywords: Confocal microscope, Filter, Flow cytometry, Fluorescence Correlation Spectroscopy (FCS), Fluorescence Lifetime Imaging Microscopy (FLIM), Fluorescence localization after photobleaching (FLAP), Fluorescence loss in photobleaching (FLIP), Fluorescence microscope, Fluorescence photobleaching, Fluorescence recovery after photobleaching (FRAP), Imaging system, Light-sheet microscope, Microscope objective, Time-resolved microscopy, Total Internal Reflection Fluorescence Microscopy (TIRFM).