Evaluation of Synthetic Cell-Penetrating Peptides, Pro-Rich Peptide and Octaargine Derivatives, as Adenovirus Vector Carrier

Title: Evaluation of Synthetic Cell-Penetrating Peptides, Pro-Rich Peptide and Octaargine Derivatives, as Adenovirus Vector Carrier

Volume: 17 Issue: 2

Author(s): Shinya Kida, Yusuke Eto, Yasuo Yoshioka, Shinsaku Nakagawa, Koichi Kawasaki and Mitsuko Maeda

Affiliation:

Keywords: Adenovirus, Pro-rich peptide, octaarginine, adenovirus vector, peptide synthesis, cell-penetrating peptide

Abstract: Two cell-penetrating peptides, a Pro-rich peptide derivative, acetyl-(Val-Arg-Leu-Pro-Pro-Pro)3-Gly-Cys amide, and an octaarginine derivative, acetyl-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Gly-Cys amide, were prepared by the solid phase method. Each peptide was coupled to the heterobifunctional cross-linking reagent, 6-maleimidohexanoic acid N-hydroxysuccinimide ester, and then conjugated to the Adenovirus vector containing luciferase gene. Peptide-modified Ad, as compared with wild-type Ad, exhibited excellent luciferase activity in B16BL6 cells.

Cite this article as:

Kida Shinya, Eto Yusuke, Yoshioka Yasuo, Nakagawa Shinsaku, Kawasaki Koichi and Maeda Mitsuko, Evaluation of Synthetic Cell-Penetrating Peptides, Pro-Rich Peptide and Octaargine Derivatives, as Adenovirus Vector Carrier, Protein & Peptide Letters 2010; 17 (2) . https://dx.doi.org/10.2174/092986610790225978