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Research Article

白藜芦醇通过调节外泌体分泌抑制肝癌进展

卷 31, 期 15, 2024

发表于: 21 September, 2023

页: [2107 - 2118] 页: 12

弟呕挨: 10.2174/0929867331666230914090053

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摘要

背景与目的:白藜芦醇是一种很有前途的肿瘤治疗药物，但其抗肿瘤机制尚不清楚。本研究旨在探讨白藜芦醇对外泌体分泌的影响，以及白藜芦醇诱导的外泌体在肝细胞癌进展中的作用。 方法:采用纳米颗粒跟踪法和高通量测序法分别测定白藜芦醇诱导Huh7细胞外泌体的数量和含量。Western blotting和免疫荧光法检测Rab27a的表达。在白藜芦醇和外泌体的刺激下，观察细胞的增殖、迁移和上皮-间质转化，用Western blot和免疫荧光法检测白藜芦醇诱导的外泌体诱导的细胞自噬和wnt/β-catenin信号的活性以及lncRNA SNHG29的表达。 结果:发现白藜芦醇可能通过下调Rab27a的表达抑制外泌体分泌，从而抑制Huh7细胞的增殖、迁移和上皮间质转化。此外，白藜芦醇诱导的外泌体还可以通过抑制β-catenin的核易位和自噬的激活来抑制Huh7细胞的恶性表型，而lncRNA SNHG29可能介导了这一过程。 结论:白藜芦醇通过调节外泌体分泌和含量抑制肝癌进展。

关键词: 肝细胞癌，白藜芦醇，外泌体，自噬，lncRNA，纳米颗粒。

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