Quantification Tests for Carbohydrate Detection

Pp: 32-54 (23)

* (Excluding Mailing and Handling)

Abstract

Quantification of carbohydrates by the anthrone method is a colourimetric assay. In this method, first, the complex carbohydrates are hydrolysed in a highly acidic medium, resulting in monosaccharides. Thereafter, these monosaccharides are dehydrated to form 5-hydroxymethylfurfural (5-HMF) or 2-furfural (2-F), followed by reacting with anthrone reagent. This reaction produces a blue-green complex which is colourimetrically determined. In DNSA method 3,5-dinitrosalicylic acid (DNSA), a yellow colour reagent reacts with reducing sugar’s carbonyl group and forms orangered colour compound 3-Amino-5-nitrosalicylic acid (ANSA). The quantity of carbohydrate present in a sample is determined by Beer-Lambert’s law. Apart from these techniques Folin-Wu, Hexokinase, Phenol-Sulphuric acid, Somogvi-Nelson, and GOPOD method are discussed in this chapter in detail.

Keywords: Anthrone, Carbohydrate, Colourimetry, Folin-wu, GOPOD, Hexokinase, Phenol-sulphuric acid, Somogvi-Nelson, 5-hydroxymethylfurfural (5-HMF), 2-furfural (2-F), 3, 5-dinitrosalicylic acid (DNSA), 3-Amino5-nitrosalicylic acid (ANSA).