Stem Cells-Based Technological Innovation in Tissue Engineering

Pp: 144-159 (16)

* (Excluding Mailing and Handling)

Abstract

Stem cells are a category of cells with self-renewal and multi-lineage differentiation capacity, which have been recognized as advantaged sources for tissue engineering and regenerative medicine. To date, stem cells and their derivatives alone or combined with biomaterials have aroused extensive and sustained attention to investigations in the field of fundamental research and clinical practice. In recent years, a series of novel technologies have been involved in stem cell-based cytotherapy, such as three-dimensional (3D) printing, organoid research, and multitudinous kinds of gene-editing technologies, which collectively facilitate the development of tissue engineering for disease administration. In this chapter, we summarized the rudimentary knowledge of the aforementioned new technologies, together with the promising perspective and the concomitant challenges, which would help increase the cognition of technological innovation for stem cell-based investigations and remedies in the future.

Keywords: Bioprinting products, CRISPR/Cas9, CAR-T, Deoxyribonucleic acid, Gene knock-out, Gene knock-in, Human embryonic stem cells, Human induced pluripotent stem cells, In vivo organogenesis, Kidney organoid, Lipid nanoparticles, Modeling diseases, Nolecular mechanisms, Organoids, Single-cell transcriptomics, Stem cells, Scaffolds, TALENs, Three-dimensional printing, Tissue engineering.