Abstract
This chapter briefly discusses the general aspects that can affect the result of
TLC. Sample preparation, application of samples, preparation of development solvents,
the steps involved in the development of TLC, visualization and derivatization, and
detection of analyte are the key steps of chromatographic development. This chapter
focuses on the process steps for HPTLC analysis.
Keywords: Derivatization, Detection, Stationary phase, Visualization.
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