The sdLDL Reduces MRC1 Expression Level and Secretion of Histamin e in Differentiated M2-macrophages from Patients with Coronary Artery Stenosis

Title:The sdLDL Reduces MRC1 Expression Level and Secretion of Histamin e in Differentiated M2-macrophages from Patients with Coronary Artery Stenosis

Volume: 17 Issue: 1

Author(s): Ameneh Yarnazari, Parisa Hassanpour, Syed Reza Hosseini-Fard, Abdollah Amirfarhangi and Mohammad Najafi*

Affiliation:

• Biochemistry Department, Cellular and Molecular Research Center, University of Medical Sciences, Tehran,Iran

Keywords: Macrophage, histamine, sdLDL, stenosis, atherosclerosis, coronary arteries.

Abstract: Background: The macrophage polarization is proposed to be involved in initial events and remodeling of atherosclerosis plaques. Mannose receptor, C type 1 (MRC1) is a trans-membrane glycoprotein participating in phagocytosis and, is highly expressed in the M2 macrophages.

Objective: The aim of this study was to investigate the effects of sdLDL (small dense LDL) on the MRC1 gene expression level and secretion of histamine in the differentiated M2 macrophages from monocytes of patients with coronary artery stenosis and healthy subjects.

Method: The monocytes were isolated from healthy subjects (< 5% stenosis) and patients (>70% stenosis, SVD (Single Vessel Disease), 2VD (Two-Vessel Disease) and 3VD (Three-Vessel Disease)) by RosetteSep kit and, were differentiated into M2 macrophages by macrophage colonystimulating factor (M-CSF). The sdLDL particles were obtained by PEG-combined precipitation method. The MRC1 gene expression and histamine levels were measured by RT-qPCR and ELISA techniques, respectively.

Results: The MRC1 gene expression level was significantly increased in M2 macrophages of healthy subjects (P=0.05) while it reduced in SVD (P=0.05), 2VD (P=0.01) and 3VD (P=0.9) patients after treatment with sdLDL. The histamine value secreted from M2 macrophages (7-day) was higher (>3-fold, P=0.02) in patients as compared to healthy controls.

Conclusion: The results showed that the sdLDL particles reduce the MRC1 gene expression levels in the differentiated M2 macrophages from patients with coronary artery disease. Furthermore, they had high inflammatory capacity for the secretion of histamine.

Cite this article as:

Yarnazari Ameneh, Hassanpour Parisa, Hosseini-Fard Reza Syed, Amirfarhangi Abdollah and Najafi Mohammad*, The sdLDL Reduces MRC1 Expression Level and Secretion of Histamin e in Differentiated M2-macrophages from Patients with Coronary Artery Stenosis, Cardiovascular & Hematological Disorders-Drug Targets 2017; 17 (1) . https://dx.doi.org/10.2174/1871529X17666170106095554

DOI https://dx.doi.org/10.2174/1871529X17666170106095554	Print ISSN 1871-529X
Publisher Name Bentham Science Publisher	Online ISSN 2212-4063