Caffeic Acid Phenethyl Ester Restores Adipocyte Gene Profile Expression Following Lipopolysaccharide Treatment

Title:Caffeic Acid Phenethyl Ester Restores Adipocyte Gene Profile Expression Following Lipopolysaccharide Treatment

Volume: 14 Issue: 4

Author(s): Ignazio Barbagallo, Giovanni Li Volti, Valeria Sorrenti, Claudia Di Giacomo, Rosaria Acquaviva, Marco Raffaele, Fabio Galvano and Luca Vanella

Affiliation:

Keywords: Adipocyte, heme oxygenase, inflammation, caffeic acid phenethyl ester, antioxidants.

Abstract: Background: Obesity alters endocrine and metabolic function of the adipose tissue leading to an increased release of fatty acids, adipokynes and pro-inflammatory molecules which in turn lead to complications related to the onset of metabolic syndrome.

Methods: To this regard, natural antioxidants have been found to have anti-inflammatory and protective action in cardiovascular diseases, diabetes mellitus and obesity. Caffeic acid phenethyl ester (CAPE) is synthesized in a variety of plants and is one of the main components of propolis. CAPE inhibits oxidative stress and showed beneficial effects under various experimental conditions.

Aims: The aim of the study was to evaluate the effect of CAPE on adipocyte function after an inflammatory stimulus with lipopolysaccharide (LPS) (1 ng/ml for 6h).

Results: Our data showed that LPS caused an increased expression of proinflammatory interleukin IL-6 and a lipolytic effect on the adipocytes. CAPE treatment inhibited LPS effects through a significant decrease of IL-6 and a concomitant increase of peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT/Enhancer Binding Protein alpha (CEBPα), fatty acid synthase (FAS), fatty acid binding protein 4 (FABP4), diacylglycerol O-acyltransferase 1 (DGAT1) and transcription factor sterol regulatory element binding protein-1c (SREBP-1c). Moreover, the increased levels of adiponectin, heme oxygenase-1, nuclear factor (erythroid-derived 2)-like 2 (Nrf2), peroxisome proliferator- activated receptor alpha (PPARα) and Sirtuin-1 suggest that CAPE administration restores adipocyte function following inflammation.

Conclusion: In conclusion, the use of this metabolite such as a food supplement may represent a possible strategy for the treatment of conditions related to metabolic syndrome.

Cite this article as:

Barbagallo Ignazio, Volti Li Giovanni, Sorrenti Valeria, Giacomo Di Claudia, Acquaviva Rosaria, Raffaele Marco, Galvano Fabio and Vanella Luca, Caffeic Acid Phenethyl Ester Restores Adipocyte Gene Profile Expression Following Lipopolysaccharide Treatment, Letters in Drug Design & Discovery 2017; 14 (4) . https://dx.doi.org/10.2174/1570180813666160901124707

DOI https://dx.doi.org/10.2174/1570180813666160901124707	Print ISSN 1570-1808
Publisher Name Bentham Science Publisher	Online ISSN 1875-628X