Non-Viral Methods For Generating Integration-Free, Induced Pluripotent Stem Cells

Title:Non-Viral Methods For Generating Integration-Free, Induced Pluripotent Stem Cells

Volume: 10 Issue: 2

Author(s): Xiao-Yue Deng, Hu Wang, Tao Wang, Xian-Tao Fang, Li-Li Zou, Zhi-Ying Li and Chang-Bai Liu

Affiliation:

Keywords: DNA integration-free, induced pluripotent stem cells, non-viral methods.

Abstract: Induced pluripotent stem (iPS) cells were created from mouse fibroblasts by induced expression of Yamanaka factors, Oct3/4, Sox2, Klf4, and c-Myc. This technique has quickly resulted in an exponential increase in the amount of pluripotency studies, and has provided a valuable tool in regenerative medicine. At the same time, many methodologies to generate iPS cells have been reported, and are comprised mainly of viral methods and non-viral methods. Although viral methods may not be applicable for clinical applications, various nonviral methods have been reported in recent years, including DNA vector-based approaches, transfection of mRNA, transduction of reprogramming proteins, and use of small molecule compounds. This review summarizes and evaluates these non-viral methods.

Cite this article as:

Deng Xiao-Yue, Wang Hu, Wang Tao, Fang Xian-Tao, Zou Li-Li, Li Zhi-Ying and Liu Chang-Bai, Non-Viral Methods For Generating Integration-Free, Induced Pluripotent Stem Cells, Current Stem Cell Research & Therapy 2015; 10 (2) . https://dx.doi.org/10.2174/1574888X09666140923101914