Abstract
Cholesterol oxidase (COX, E.C.1.1.3.6), catalyses the oxidation of cholesterol to 4-cholesten-3-one with the reduction of oxygen to hydrogen peroxide. Qualitative analysis of bacterial strain (Bacillus sp. BT COX-T3) gave Gram positive rod-shaped colonies with high cholesterol degradation. Organic solvent tolerant microorganisms are novel group of extremophilic microorganisms that have developed resistance to withstand solvent toxicity. These organisms play an important role in biotransansformation and bioconversion of organic compounds. An organic solvent-tolerant Bacillus sp. BT COX-T3 obtained after primary screening for production of extracellular COX transformed cholesterol to 4-cholesten- 3-one in toluene, and resulted in a higher COX production in a biphasic medium due to an increase in solubility of cholesterol in the medium making it easily available to be metabolized by the organism. A simple screening method for 6β-hydroperoxycholest-4-en-3-one formed by Bacillus sp. COX-T3 was devised in this study and bioconversion was monitored spectrophotometerically. Presence of cholesterol in 20% (v/v) toluene was observed to be most effective biotransformation system for bacterial COX production. A maximum COX activity of 0.892 U/ml was obtained at 45°C by using 8% (v/v) inoculum, 0.1% (w/v) cholesterol as the main carbon source and 0.5% (w/v) yeast extract. Amongst various salt ions only Co2+ ions improved the COX production by Bacillus sp. COX-T3. Moreover, the cholesterol provided in the bioconversion system was completely transformed by Bacillus sp. COX-T3. Bacillus sp COX-T3 effectively degraded cholesterol in the presence of toluene and the extracellular COX produced by it was highly stable at the end of 144 h.
Keywords: Cholesterol, cholesterol oxidase, bioconversion, solvent tolerant.
Call for Papers in Thematic Issues
Age Reversal using transient epigenetc reprogramming with the OSK Yamanaka Factors
Aging is a complex biological process characterized by a gradual deterioration of physiological functions and an increased vulnerability to disease. Recent advancements in the field of regenerative medicine have shown promising avenues for combating aging. Among these, partial epigenetic reprogramming, specifically utilizing the Yamanaka factors, has been shown to reverse ...read more
Bioinformatic Analysis of Regulated Cell Death in Chronic and Acute Inflammatory Diseases
In the intricate realm of inflammatory diseases, delving into the complexities surrounding regulated cell death emerges as an indispensable and forefront area of exploration. Understanding the intricacies of how cells are regulated to undergo death in inflammatory conditions not only represents a pivotal frontier in biomedical research but also holds ...read more
Feeding a vegan World
The Eat-Lancet Planetary diet recommends at least 2/3 plant and microbial sources in the food protein portion. Such diet is recommended against obesity, cancer and to limit global warming, and might get more and more incentives in the next years. We thus need massive amounts of Plant-based food, and the ...read more
Leveraging AI for personalized pediatric neurodevelopmental support
Personalized medicine in pediatrics is a moving target dedicated to finding the right treatment, at the right time, for each individual child in each stage of development. Child development is a highly personal process, unique to each individual, something which is even more apparent in the development of children with ...read more
Related Journals
Related Books
Opportunities for Biotechnology Research and Entrepreneurship
Research Methodology and Project Management in Biotechnology
Recent Progress in Pharmaceutical Nanobiotechnology: A Medical Perspective
Recent Trends In Livestock Innovative Technologies
Algal Biotechnology for Fuel Applications
Terpenoids: Recent Advances in Extraction, Biochemistry and Biotechnology
Recent Advances in Biotechnology
2
