Abstract
White grape pomace, containing residual fermentable sugar, has been evaluated as a substrate for production of butanol by submerged fermentation using Clostridium saccharobutylicum P262. Using an initial value of pH 5.5, an inoculum size of 10% (v/v) and addition of supplementary yeast extract (10 g/l), butanol concentrations of up to 6 g/l were obtained, at a yield, based on sugar utilized, of 0.2 g/g. Significantly, the requirement for supplementary yeast extract could be replaced by selected salts, indicating that white grape pomace is not lacking in any organic nutrients for growth of, and butanol production by, C. saccharobutylicum, and hence has potential commercial application.
Keywords: ABE fermentation, butanol, Clostridium saccharobutylicum, grape pomace, submerged fermentation, biofuel, yeast extract supplementation, nitrogen salts, inoculum concentration, butanol yield, butanol productivity, vineyard waste, Chardonnay pomace, pH, fermentable sugars, strain P262, fermentation profile.
Current Biotechnology
Title:Butanol Production by Submerged Fermentation of White Grape Pomace
Volume: 2 Issue: 2
Author(s): Laurent Law and Noemi Gutierrez
Affiliation:
Keywords: ABE fermentation, butanol, Clostridium saccharobutylicum, grape pomace, submerged fermentation, biofuel, yeast extract supplementation, nitrogen salts, inoculum concentration, butanol yield, butanol productivity, vineyard waste, Chardonnay pomace, pH, fermentable sugars, strain P262, fermentation profile.
Abstract: White grape pomace, containing residual fermentable sugar, has been evaluated as a substrate for production of butanol by submerged fermentation using Clostridium saccharobutylicum P262. Using an initial value of pH 5.5, an inoculum size of 10% (v/v) and addition of supplementary yeast extract (10 g/l), butanol concentrations of up to 6 g/l were obtained, at a yield, based on sugar utilized, of 0.2 g/g. Significantly, the requirement for supplementary yeast extract could be replaced by selected salts, indicating that white grape pomace is not lacking in any organic nutrients for growth of, and butanol production by, C. saccharobutylicum, and hence has potential commercial application.
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Cite this article as:
Law Laurent and Gutierrez Noemi, Butanol Production by Submerged Fermentation of White Grape Pomace, Current Biotechnology 2013; 2 (2) . https://dx.doi.org/10.2174/22115501113029990003
DOI https://dx.doi.org/10.2174/22115501113029990003 |
Print ISSN 2211-5501 |
Publisher Name Bentham Science Publisher |
Online ISSN 2211-551X |
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