Biogenic Silver Nanoparticles (AgNPs) Ameliorates Oxidative Biomarkers in Type-2 Diabetic Rats: In vitro and In vivo Report

Title:Biogenic Silver Nanoparticles (AgNPs) Ameliorates Oxidative Biomarkers in Type-2 Diabetic Rats: In vitro and In vivo Report

Volume: 18 Issue: 2

Author(s): Gideon Ayeni*, Larayetan Abisoye Rotimi, Yahaya Abdulrazaq, Emmanuel F. Titus, Sule Fatima Ajuma, Audu Omale Jamila, Ajayi Abayomi, Egwumah Christian, Ogohi Dorathy Agah, Ogundipe Emmanuel, Audu Umar Faruk, Orogun Mary Moradeke, Monday Sylvester, Ogejah Ize Zuleihat, Wulapba Nenpinmwa Dauda and Achimi Matthew Akoji

Affiliation:

• Department of Biochemistry, Prince Abubakar Audu University, Anyigba, Kogi State, PMB 1008, Nigeria

Keywords: Nanoparticles, T. tetrapleura, spectroscopy, streptozotocin, type 2- diabetes, inhibition, scavenging.

Abstract:

Objective: Nanoparticles (NPs) are reliable biological tools for curative purposes through their application in nanomedicine. The present study synthesized and characterized silver nanoparticles (AgNPs) from Tetrapetra tetrapleura fruit. The investigation aims to examine the antidiabetic effect of the AgNPs using in vitro and in vivo models.

Methods: Briefly, the synthesized AgNPs were confirmed by the application of ultraviolet-visible (UV-Vis) spectroscopy, and five other techniques, viz; transmission electron microscopy (TEM) techniques, Fourier transform infrared (FTIR) spectroscopy, energy dispersive X-ray spectroscopy (EDX), X-ray diffraction analysis (XRD) and scanning electron microscope (SEM). The in vitro model assay investigated the scavenging effect of AgNPS on 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide anion (O2ˉ), hydroxyl anion (-OH), ferric reducing antioxidant power (FRAP), and α-amylase/α-glucosidase inhibitory activity. Thein vivo model involving rats-induced type-2 diabetes with streptozotocin (STZ) was divided into six (6) groups of seven (7) rats each to assess antioxidative parameters.

Results: The AgNPs scavenged free radicals (DPPH) and moderately inhibited (O2ˉ), hydroxyl anion (-OH), reduced ferric to ferrous ions, and inhibited both α-amylase and α-glucosidase activity with increasing concentrations. Similarly, AgNPs ameliorated oxidative stress imposed by type 2 diabetes on the rats’ tissues significantly (p < 0.05), depleting total cholesterol, low-density lipoprotein (LDL), and increased total protein composite and high-density lipoprotein (HDL) contents. The AgNPs enhanced catalase and superoxide dismutase, reduced glutathione (GSH), and, concomitantly, decreased malondialdehyde (MDA) levels in the tissue homogenate.

Conclusion: These findings provide scientific evidence for the first time, finding the application of a biogenic compound synthesized from T. tetrapleura fruit in the treatment of type 2 diabetes.

Cite this article as:

Ayeni Gideon*, Rotimi Abisoye Larayetan, Abdulrazaq Yahaya, Titus F. Emmanuel, Ajuma Fatima Sule, Jamila Omale Audu, Abayomi Ajayi, Christian Egwumah, Agah Dorathy Ogohi, Emmanuel Ogundipe, Faruk Umar Audu, Moradeke Mary Orogun, Sylvester Monday, Zuleihat Ize Ogejah, Dauda Nenpinmwa Wulapba and Akoji Matthew Achimi, Biogenic Silver Nanoparticles (AgNPs) Ameliorates Oxidative Biomarkers in Type-2 Diabetic Rats: In vitro and In vivo Report, Current Chemical Biology 2024; 18 (2) . https://dx.doi.org/10.2174/0122127968284847240920103341