Identification of Two HIV-1 CRF01_AE/B Recombinant Forms and a CRF01_AE/B/C Recombinant Form in Hebei Province, China

Title:Identification of Two HIV-1 CRF01_AE/B Recombinant Forms and a CRF01_AE/B/C Recombinant Form in Hebei Province, China

Volume: 22 Issue: 5

Author(s): Xiangyun Tang, Meng Liu, Ning An, Xinyu Zhang, Yingying Wang, Yan Li and Xinli Lu*

Affiliation:

• Department of AIDS Research, Hebei Key Laboratory of Pathogen and Epidemiology of Infectious Disease, Hebei Provincial Center for Disease Control and Prevention , Shijiazhuang, Hebei, 050021, China
• School of Public Health, North China University of Science and Technology, Tangshan, Hebei, 063210, China
• School of Public Health, Hebei Medical University, Shijiazhuang, Hebei, 050017, China

Keywords: HIV-1, novel recombination forms, AIDS, NFLGs, phylogenetic trees and breakpoints analysis, MSM.

Abstract:

Background: In the Hebei province, Human Immunodeficiency Virus type one (HIV-1) recombinant strains of subtypes B, C, and CRF01_AE are emerging very rapidly and diversely.

Objective: In order to confirm the characteristics of novel recombination forms, we aimed to analyze HIV-1 Near-full-length Genome sequences (NFLGs) obtained from three Men who have Sex with Men (MSM) in this study.

Methods: Phylogenetic trees were constructed and breakpoints analysis were performed based on the NFLGs and each gene fragment to examine the gene recombination patterns of three new HIV-1 NFLGs.

Results: HIV-1 subtypes CRF01_AE and B were combined to generate the recombinant structures of the NFLGs 610 and 687. CRF01_AE, B, and C were combined to generate the recombinant structures of the NFLG 825. According to the NFLG phylogenetic tree, the NFLG 825 clustered with CRF65_cpx and the NFLGs 610 and 687 clustered with CRF68_01B. The recombination breakpoints analysis revealed that the recombination pattern of the NFLGs 610 and 687 was the insertion of subtype B fragment into the CRF01_AE backbone. Subregions I, II, and III were derived from CRF01_AE, subtype B, and CRF01_AE, respectively. The recombination pattern of the NFLG 825 contained ten fragments of subtypes CRF01_AE, C, and B. Finally, the above factors were formed using phylogenetic trees and breakpoints analysis, which were combined to get two CRF68_01B forms and one CRF65_cpx form.

Conclusion: Our findings have suggested that it is crucial to keep an eye on the genetic diversity of HIV-1 in Hebei province.

Cite this article as:

Tang Xiangyun, Liu Meng, An Ning, Zhang Xinyu, Wang Yingying, Li Yan and Lu Xinli*, Identification of Two HIV-1 CRF01_AE/B Recombinant Forms and a CRF01_AE/B/C Recombinant Form in Hebei Province, China, Current HIV Research 2024; 22 (5) . https://dx.doi.org/10.2174/011570162X294091240812062836