Evaluation of Fluctuations of BRAF Gene Expression and its Polymorphism at rs1267623 in Colorectal Cancer

Title:Evaluation of Fluctuations of BRAF Gene Expression and its Polymorphism at rs1267623 in Colorectal Cancer

Volume: 13 Issue: 3

Author(s): Seyedeh Elham Norollahi, Kosar Babaei, Sogand Vahidi, Seyed Javad Hosseini Motaz, Mahmoud Kalani Tarbeghan, Mostafa Khaleghipour, Elaheh Asghari Gharakhyli, Seyed Reza Mirhafez*Ali Akbar Samadani*

Affiliation:

• Noncommunicable Diseases Research Center, Neyshabur University of Medical Sciences, Neyshabur,

• Noncommunicable Diseases Research Center, Neyshabur University of Medical Sciences, Neyshabur,
• Guilan Road Trauma Research Center, Trauma Institute, Guilan University of Medical Sciences, Rasht, Iran

Keywords: Colorectal cancer, BRAF gene expression, polymorphism, biomarker, mutation, diagnosis.

Abstract:

Background: Molecular markers in Colorectal Cancer (CRC) are needed for more accurate classification and personalized treatment. In this way, we investigated the effects of the BRAF gene on clinical outcomes of its expression fluctuations and its polymorphism at rs1267623 in CRC.

Methods: In this study, 36.36 percent of patients with CRC were women, and 63.63 percent were men. After the pathology department confirmed the tumor of the samples, the stage and grade of the tumor were determined according to the TNM system. Real-time PCR was used to check the expression of the BRAF gene in tumor and non-tumor tissues, and its polymorphism in rs1267623 was also checked using the Tetra-ARMs PCR technique.

Results: The expression of BRAF in tumor tissues was significantly higher than in non-tumoral tissues (P = 0.001), indicating an upregulation of BRAF gene expression in tumoral tissues. The user's text is empty. Furthermore, there was a significant correlation between BRAF expression and tumor stage (P= 0.001), as well as tumor grade (P = 0.003). However, no significant link was found between lymph node metastasis and distant metastasis of BRAF gene expression (P = 0.3). Additionally, no mutation was detected in the investigation of rs1267623 polymorphism.

Conclusion: The BRAF gene was upregulated in tumoral tissues. Remarkably, no mutation was found in the rs1267623 polymorphism. As a result, this gene can be used as a biomarker in the diagnosis and treatment of CRC.

Cite this article as:

Norollahi Elham Seyedeh, Babaei Kosar, Vahidi Sogand, Motaz Javad Hosseini Seyed, Tarbeghan Kalani Mahmoud, Khaleghipour Mostafa, Gharakhyli Asghari Elaheh, Mirhafez Reza Seyed*, Samadani Akbar Ali*, Evaluation of Fluctuations of BRAF Gene Expression and its Polymorphism at rs1267623 in Colorectal Cancer, MicroRNA 2024; 13 (3) . https://dx.doi.org/10.2174/0122115366286360240625095932