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Review Article

Crucial Role of microRNAs as New Targets for Amelogenesis Disorders Detection

卷 24, 期 14, 2023

发表于: 05 November, 2023

页: [1139 - 1149] 页: 11

弟呕挨: 10.2174/0113894501257011231030161427

价格: $65

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摘要

介绍：釉质发育不全(AI)是指由多种因素导致的釉质低矿化的异质组。预防措施对于预测这种病理是必要的。预防医学的前景与寻找诊断人类疾病的新的信息方法密切相关。MicroRNAs是非侵入性诊断平台的重要组成部分。 研究目的：回顾的目的是回顾参与成淀粉性发育的异质因素，并选择与AI类型相关的microRNA面板。 方法：我们使用DIANA Tools(算法、数据库和软件)在一个系统框架中解释和归档数据，从深度测序数据的表达调控分析到miRNA调控元件和靶点的注释(https://dianalab)。e-ce.uth.gr /)。在我们的研究中，基于与AI类型相关的基因面板，鉴定出24个miRNA用于发育不良型(补充)，35个用于低钙化型，49个用于低饱和度AI。选择策略包括使用AI类型的基因面板对多个靶标进行microRNA搜索。 结果：分析了关键蛋白、钙依赖因子和遗传因子，揭示了它们在成胚发生中的作用。具有多种调控功能的细胞外非编码RNA序列似乎是最具吸引力的。我们选择了与AI基因相关的microrna列表。我们发现了四种microrna (hsa-miR-27a-3p, hsa-miR-375, hsa-miR-16-5p和hsamiR- 146a-5p)的基因面板，与发育不良型AI相关;5个microrna (hsa- miR-29c-3p、hsa- mir -124-3p、hsa- mir -1343-3p、hsa- mir -335-5p和hsa- mir -16-5p)用于低钙化型AI, 7个microrna (hsa- mir -124-3p、hsa- mir -147a、hsa- mir -16-5p、hsamiR- 429、hsa- lett -7b-5p、hsa- mir -146a-5p、hsa- mir -335-5p)用于低饱和度AI。结果显示，hsa-miR-16-5p包含在发育不良、低钙化和低饱和度类型的三个特异性面板中。Hsa-miR-146a-5p与AI发育不良和低饱和度相关，这与炎症反应免疫反应的特殊性有关。反过来，hsa-miR-335-5p与低钙化和低饱和型AI相关。 结论：液体活检方法是一种有希望的方法，以减少治疗这些患者在现代医疗保健的经济成本。关于microRNA在调节成淀粉性发育中的作用存在独特的数据。与人工智能基因相关并按人工智能类型分类的microrna列表已经被发现。靶基因分析显示了所选择的microrna的多种功能，这解释了成胚发生的多种异质机制。对矿化问题的倾向是一个程序化的事件。许多因素决定了这个问题的表现。此外，有必要记住变化的可变性质，这降低了预测的准确性。因此，基于液体活检和microrna的模型可以考虑这些因素及其对矿化的影响。发现的数据需要进一步调查。

关键词: 无体发育不全，遗传因素，生长因素，钙代谢，microRNA，液体活检。

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