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General Research Article

不同癌症患者金属催化氧化损伤DNA自身抗体的产生

卷 31, 期 5, 2024

发表于: 09 June, 2023

页: [640 - 648] 页: 9

弟呕挨: 10.2174/0929867330666230503143133

价格: $65

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摘要

背景：自由基是一种不稳定的高活性物质，存在于体内和体外。自由基被标记为由代谢和内源性氧燃烧形成的电子饥渴分子。它们在细胞内运输，扰乱分子的排列，引发细胞损伤。羟基自由基(•OH)是一种高活性自由基，对其附近的生物分子具有损伤作用。 方法：在本研究中，DNA被通过芬顿反应产生的羟基自由基修饰。用紫外可见光谱和荧光光谱对氧化修饰DNA (Ox-DNA)进行了表征。热变性是为了揭示修饰DNA对热的敏感性。通过直接结合ELISA检测癌症患者血清中Ox-DNA自身抗体的存在，也证实了Ox-DNA的作用。同时用抑制酶联免疫吸附试验检测自身抗体的特异性。 结果：在生物物理表征中，与天然DNA类似物相比，报告了Ox-DNA的高染度增加和荧光强度的相对降低。一项热变性研究表明，与天然构象相比，Ox-DNA对热非常敏感。直接结合酶联免疫吸附试验显示，从癌症患者血清中分离出针对Ox-DNA的自身抗体。生成的Ox-DNA自身抗体对膀胱癌、头颈癌和肺癌具有高度特异性，血清抗体和IgG抗体的抑制ELISA进一步证实了这一点。 结论：DNA分子上产生的新表位被免疫系统识别为非自身，从而导致癌症患者形成自身抗体。因此，我们的研究证实了氧化应激在DNA的结构扰动中起作用，使其具有免疫原性。

关键词: 羟基自由基(•OH)，芬顿反应，新表位，自身抗体，癌症，受损DNA。

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