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Current Neurovascular Research

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ISSN (Print): 1567-2026
ISSN (Online): 1875-5739

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Research Article

Cerium Oxide Nanoparticles Ameliorate Oxidative Stress, Inflammation, and Pain Behavior in Neuropathic Rats

Author(s): Fatemeh Forouzanfar*, Ali Mohammad Pourbagher-Shahri, Majid Darroudi, Mahmood Sadeghi, Farzaneh Vafaee, Omid Fakharzadeh Moghadam, Negar Moghaddas Mashhad, Hamed Ghazavi and Mohammad Bagher Khorrami

Volume 20, Issue 1, 2023

Published on: 22 February, 2023

Page: [54 - 61] Pages: 8

DOI: 10.2174/1567202620666230125104604

Price: $65

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Abstract

Background: Neuropathic pain originating from a dysfunction in the nervous system is often intractable and chronic. Recently, several studies using nanoparticles suggested a new way to control neuropathic pain. This study intended to explore the potential neuroprotective effect of Cerium Oxide Nanoparticles (CNPs) synthesized by pullulan in neuropathic pain in rats.

Methods: On the right common sciatic nerve of male Wistar rats, the chronic constriction injury (CCI) procedure was used to establish a neuropathic pain model. CNPs were injected into the caudal vein of the rat. Behavioral methods were used to detect mechanical allodynia, cold allodynia, and thermal hyperalgesia in rats. Besides, inflammation factors, including tumor necrosis factor (TNF)-α, interleukin (IL)-1β, nitric oxide (NO), and markers of oxidative stress, including Malondialdehyde (MDA) and total thiol, were measured in the spinal cord segment of rats.

Results: In rats with CCI, mechanical allodynia, cold allodynia, and thermal hyperalgesia developed, which improved when the rats were administered CNPs. Spinal cord specimens of CCI rats had elevated inflammation and oxidative stress status (↑IL-1β, ↑TNF-α, ↑NO, ↑MDA) and decreased antioxidative levels (↓total thiol). As a result of CNPs treatment, these changes were reversed in the spinal cord specimens.

Conclusion: CNPs alleviate neuropathic pain by exhibiting antioxidative and anti-inflammatory activities.

Keywords: Cerium oxide nanoparticles, neuropathic pain, oxidative stress, neuroinflammation, chronic pain, pullulan.

« Previous Next »
[1]
Raja SN, Carr DB, Cohen M, et al. The revised IASP definition of pain: Concepts, challenges, and compromises. Pain 2020; 161(9): 1976.
[2]
Haanpää M, Treede RD. Neuropathic pain. Clinical Pain Management: A Practical Guide 2022; 373-80.
[http://dx.doi.org/10.1002/9781119701170.ch36]
[3]
Finnerup NB, Haroutounian S, Kamerman P, et al. Neuropathic pain: an updated grading system for research and clinical practice. Pain 2016; 157(8): 1599.
[http://dx.doi.org/10.1097/j.pain.0000000000000492]
[4]
Kumar V, Sharma A. Neutrophils: Cinderella of innate immune system. Int Immunopharmacol 2010; 10(11): 1325-34.
[5]
Moalem G, Xu K, Yu LJN. T lymphocytes play a role in neuropathic pain following peripheral nerve injury in rats. Neuroscience 2004; 129(3): 767-7.
[http://dx.doi.org/10.1016/j.neuroscience.2004.08.035]
[6]
Palmer MT, Weaver CT. Autoimmunity: increasing suspects in the CD4+ T cell lineup. Nature i Immunology 2010; 11(1): 36-40.
[7]
Chen Z, Wang T, Fang Y, et al. Adjacent intact nociceptive neurons drive the acute outburst of pain following peripheral axotomy. Sci Rep 2019; 9(1): 7651.
[http://dx.doi.org/10.1038/s41598-019-44172-9] [PMID: 31113988]
[8]
Attal N, Lanteri-Minet M, Laurent B, Fermanian J, Bouhassira D. The specific disease burden of neuropathic pain: Results of a French nationwide survey. Pain 2011; 152(12): 2836-43.
[http://dx.doi.org/10.1016/j.pain.2011.09.014] [PMID: 22019149]
[9]
Finnerup NB, Attal N, Haroutounian S, et al. Pharmacotherapy for neuropathic pain in adults: a systematic review and meta-analysis. Lancet Neurol 2015; 14(2): 162-73.
[http://dx.doi.org/10.1016/S1474-4422(14)70251-0] [PMID: 25575710]
[10]
Singh KR, Nayak V, Sarkar T, Singh RP. Cerium oxide nanoparticles: properties, biosynthesis and biomedical application. RSC advances 2020; 10(45): 27194-214.
[11]
Fard JK, Jafari S, Eghbal MA. A review of molecular mechanisms involved in toxicity of nanoparticles. Adv Pharm Bull 2015; 5(4): 447.
[12]
Dhall A, Self WJA. Cerium oxide nanoparticles: a brief review of their synthesis methods and biomedical applications. Antioxidants (Basel) 2018; 7(8): 97.
[http://dx.doi.org/10.3390/antiox7080097]
[13]
Alili L, Sack M, von Montfort C, et al. Downregulation of tumor growth and invasion by redox-active nanoparticles. Antioxid Redox Signal 2013; 19(8): 765-8.
[http://dx.doi.org/10.1089/ars.2012.4831]
[14]
Qi M, Li W, Zheng X, et al. Cerium and its oxidant-based nanomaterials for antibacterial applications: a state-of-the-art review. Front Mater 2020; 7: 213.
[http://dx.doi.org/10.3389/fmats.2020.00213]
[15]
Asgharzadeh F, Hashemzadeh A, Rahmani F, et al. Cerium oxide nanoparticles acts as a novel therapeutic agent for ulcerative colitis through anti-oxidative mechanism. Life Sci 2021; 278: 119500.
[http://dx.doi.org/10.1016/j.lfs.2021.119500] [PMID: 33862111]
[16]
Kobyliak N, Virchenko O, Falalyeyeva T, et al. Cerium dioxide nanoparticles possess anti-inflammatory properties in the conditions of the obesity-associated NAFLD in rats. Biomed Pharmacother 2017; 90: 608-14.
[http://dx.doi.org/10.1016/j.biopha.2017.03.099] [PMID: 28411553]
[17]
Zhou D, Fang T, Lu LQ, Yi L. Neuroprotective potential of cerium oxide nanoparticles for focal cerebral ischemic stroke. J Huazhong Univ Sci Technolog Med Sci 2016; 36(4): 480-6.
[http://dx.doi.org/10.1007/s11596-016-1612-9]
[18]
Rekha M, Sharma CP. Pullulan as a promising biomaterial for biomedical applications: a perspective. Trends Biomater Artif Organs 2007; 20(2): 116-21. [INCOMPLETE]
[19]
Khorrami MB, Sadeghnia HR, Pasdar A, et al. Antioxidant and toxicity studies of biosynthesized cerium oxide nanoparticles in rats. Int J Nanomedicine 2019; 14: 2915.
[http://dx.doi.org/10.2147/IJN.S194192]
[20]
Nonsuwan P, Puthong S, Palaga T, Muangsin N. Novel organic/inorganic hybrid flower-like structure of selenium nanoparticles stabilized by pullulan derivatives. Carbohydr Polym 2018; 184: 9-19.
[21]
Khorrami MB, Sadeghnia HR, Pasdar A, Ghayour-Mobarhan M, Riahi-Zanjani B, Darroudi M. Role of Pullulan in preparation of ceria nanoparticles and investigation of their biological activities. J Mol Struct 2018; 1157: 127-31.
[http://dx.doi.org/10.1016/j.molstruc.2017.12.053]
[22]
Bennett GJ, Xie YK. A peripheral mononeuropathy in rat that produces disorders of pain sensation like those seen in man. Pain 1988; 33(1): 87-107.
[http://dx.doi.org/10.1016/0304-3959(88)90209-6] [PMID: 2837713]
[23]
Rakhshandeh H, Pourbagher-Shahri AM, Hasanpour M, Iranshahi M, Forouzanfar F. Effects of Capparis spinosa extract on the neuropathic pain induced by chronic constriction injury in rats. Metab Brain Dis 2022; 37(8): 2839-52.
[http://dx.doi.org/10.1007/s11011-022-01094-2] [PMID: 36222985]
[24]
Forouzanfar F, Hosseinzadeh H, Khorrami MB, Asgharzade S, Rakhshandeh H. Attenuating effect of Portulaca oleracea extract on chronic constriction injury induced neuropathic pain in rats: an evidence of anti-oxidative and anti-inflammatory effects. CNS Neurol Disord Drug Targets 2019; 18(4): 342-9.
[http://dx.doi.org/10.2174/1871527318666190314110528]
[25]
Kukkar A, Singh N, Jaggi AS. Neuropathic pain-attenuating potential of aliskiren in chronic constriction injury model in rats. J Renin Angiotensin Aldosterone Syst 2013; 14(2): 116-23.
[http://dx.doi.org/10.1177/1470320312460899] [PMID: 23087256]
[26]
Rakhshandeh H, Asgharzade S, Khorrami MB, Forouzanfar F. Protective effect of Capparis spinosa extract against focal cerebral ischemia-reperfusion injury in rats. Cent Nerv Syst Agents Med Chem 2021; 21(2): 148-53.
[http://dx.doi.org/10.2174/1871524921666210625112356]
[27]
Ilari S, Giancotti LA, Lauro F, et al. Natural antioxidant control of neuropathic pain-exploring the role of mitochondrial sirt3 pathway. Antioxidants 2020; 9(11): 1103.
[http://dx.doi.org/10.3390/antiox9111103] [PMID: 33182469]
[28]
Tu W, Yue J, Li X, Wu Q, Yang G, Li S, et al. Electroacupuncture alleviates neuropathic pain through regulating miR-206-3p Targeting BDNF after CCI. Neural Plasticity 2022; 2022
[http://dx.doi.org/10.1155/2022/1489841]
[29]
Schwartz ES, Lee I, Chung K, Mo Chung J. Oxidative stress in the spinal cord is an important contributor in capsaicin-induced mechanical secondary hyperalgesia in mice. Pain 2008; 138(3): 514-24.
[http://dx.doi.org/10.1016/j.pain.2008.01.029] [PMID: 18375065]
[30]
Rossato MF, Velloso NA, de Oliveira Ferreira AP, de Mello CF, Ferreira J. Spinal levels of nonprotein thiols are related to nociception in mice. J Pain 2010; 11(6): 545-54.
[31]
Hirst SM, Karakoti AS, Tyler RD, Sriranganathan N, Seal S, Reilly CMJS. Anti‐inflammatory properties of cerium oxide nanoparticles. Small 2009; 5(24): 2848-56.
[http://dx.doi.org/10.1002/smll.200901048]
[32]
Mittal M, Siddiqui MR, Tran K, Reddy SP, Malik AB. Reactive oxygen species in inflammation and tissue injury. Antioxid Redox Signal 2014; 20(7): 1126-67.
[http://dx.doi.org/10.1089/ars.2012.5149] [PMID: 23991888]
[33]
Levy D, Zochodne DW. NO pain: potential roles of nitric oxide in neuropathic pain. Pain Pract 2004; 4(1): 11-8.
[http://dx.doi.org/10.1111/j.1533-2500.2004.04002.x] [PMID: 17129298]
[34]
Cury Y, Picolo G, Gutierrez VP, Ferreira SH. Pain and analgesia: The dual effect of nitric oxide in the nociceptive system. Nitric Oxide 2011; 25(3): 243-54.
[http://dx.doi.org/10.1016/j.niox.2011.06.004] [PMID: 21723953]
[35]
Ellis A, Bennett DLH. Neuroinflammation and the generation of neuropathic pain. Br J Anaesth 2013; 111(1): 26-37.
[http://dx.doi.org/10.1093/bja/aet128] [PMID: 23794642]
[36]
Oliveira Júnior JOd. Portella Junior CSA, Cohen CP. Inflammatory mediators of neuropathic pain. Rev Dor 2016; 17: 35-42.
[37]
Leung L, Cahill CM. TNF-α and neuropathic pain - a review. J Neuroinflammation 2010; 7(1): 27.
[http://dx.doi.org/10.1186/1742-2094-7-27] [PMID: 20398373]
[38]
Pinho-Ribeiro FA, Verri WA Jr, Chiu IM. Nociceptor sensory neuron-immune interactions in pain and inflammation. Trends Immunol 2017; 38(1): 5-19.
[http://dx.doi.org/10.1016/j.it.2016.10.001] [PMID: 27793571]
[39]
Rekha M, Sharma CP. Pullulan as a promising biomaterial for biomedical applications: a perspective. Trends Biomater Artif Organs 2007; 20(2): 116-21.
[40]
Singh RS, Kaur N, Rana V, Kennedy JF. Pullulan: A novel molecule for biomedical applications. Carbohydr Polym 2017; 171: 102-21.
[http://dx.doi.org/10.1016/j.carbpol.2017.04.089] [PMID: 28578944]
[41]
Hegazy MAE, Maklad HM, Abd Elmonsif DA, Elnozhy FY, Alqubiea MA. The possible role of cerium oxide (CeO2) nanoparticles in prevention of neurobehavioral and neurochemical changes in 6-hydroxydopamineinduced parkinsonian disease. 2017; 53(4): 351-60.
[42]
Behroozi Z, Rahimi B, Hamblin MR, Nasirinezhad F, Janzadeh A, Ramezani F. Injection of cerium oxide nanoparticles to treat spinal cord injury in rats. J Neuropathol Exp 2022; 81(8): 635-42.
[http://dx.doi.org/10.21203/rs.3.rs-547438/v1]
[43]
Najafi R, Hosseini A, Ghaznavi H, Mehrzadi S, Sharifi AM. Neuroprotective effect of cerium oxide nanoparticles in a rat model of experimental diabetic neuropathy. Brain Res Bull 2017; 131: 117-22.
[http://dx.doi.org/10.1016/j.brainresbull.2017.03.013] [PMID: 28373151]

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