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Research Article

右美托咪定通过减少氧化和细胞凋亡来减轻家兔脊髓缺血再灌注损伤

卷 23, 期 6, 2023

发表于: 15 August, 2022

页: [569 - 577] 页: 9

弟呕挨: 10.2174/1566524022666220525142954

价格: $65

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摘要

背景：在脑缺血中，右美托咪定（DEX）可防止谷氨酸和去甲肾上腺素发生变化，增加神经传导并防止细胞凋亡，但其机制尚不清楚。 目的：本研究旨在探讨DEX对脊髓缺血再灌注损伤（SCIRI）的保护作用和作用，以及该作用是否由氧化应激和细胞凋亡介导（Bcl-2、Bax、线粒体和Caspase -3的参与）。 方法：家兔随机分为假手术组、输注/再灌注（I/R）组和DEX I/R组。通过阻塞左肾动脉尾部的主动脉 40 分钟诱导 SCIRI，然后再灌注。在夹紧前连续给予 DEX 60 分钟。评估动物的神经元功能。检查脊髓组织的 SOD 活性和 MDA 含量。通过蛋白质印迹法检测 Bcl-2、Bax 和 Caspase-3 的表达。 TUNEL染色用于细胞凋亡。 结果：随着再灌注时间的延长，DEX I/R组后肢神经功能逐渐改善，I/R组后肢神经功能逐渐恶化（与同组其他时间点相比均P<0.05）.与I/R相比，DEX降低MDA，升高SOD（P<0.01），上调Bcl-2蛋白表达（P<0.05），下调Bax表达（P<0.05），降低caspase-3表达（P<0.05），阻止神经元的组织学变化，并降低TUNEL标记的细胞凋亡指数（P <0.05）。 结论：DEX可通过改善氧化应激状态、调节凋亡相关蛋白的表达、减少神经元凋亡来减轻兔SCIRI。

关键词: 脊髓损伤，缺血再灌注损伤，右美托咪定，细胞凋亡，Bcl-2，氧化应激。

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