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Current Topics in Medicinal Chemistry

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ISSN (Print): 1568-0266
ISSN (Online): 1873-4294

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Mini-Review Article

Rapid Nucleic Acid Reaction Circuits for Point-of-care Diagnosis of Diseases

Author(s): Ezry Santiago-McRae, Sung Won Oh, Anthony Monte Carlo, Omri Bar, Emily Guan, Doris Zheng, Catherine Grgicak and Jinglin Fu*

Volume 22, Issue 8, 2022

Published on: 14 March, 2022

Page: [686 - 698] Pages: 13

DOI: 10.2174/1570163819666220207114148

Price: $65

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Abstract

An urgent need exists for a rapid, cost-effective, facile, and reliable nucleic acid assay for mass screening to control and prevent the spread of emerging pandemic diseases. This urgent need is not fully met by current diagnostic tools. In this review, we summarize the current state-of-the-art research in novel nucleic acid amplification and detection that could be applied to point-of-care (POC) diagnosis and mass screening of diseases. The critical technological breakthroughs will be discussed for their advantages and disadvantages. Finally, we will discuss the future challenges of developing nucleic acid-based POC diagnosis.

Keywords: Nucleic acid assay, Isothermal amplification, Nucleic acid circuits, CRISPR-Cas, Point-of-care, Diseases.

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