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Research Article

一种识别线粒体DNA控制区甲基化位点的新方法

卷 21, 期 2, 2021

发表于: 28 May, 2020

页: [151 - 164] 页: 14

弟呕挨: 10.2174/1566524020666200528154005

价格: $65

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摘要

线粒体DNA（mtDNA）甲基化有潜力用作人类发育或疾病的生物标记。但是，mtDNA甲基化步骤既昂贵又费时。因此，我们开发了一种基于RT-PCR分析的新方法，可通过一种简单，快速，特异且低成本的策略来鉴定mtDNA控制区域中甲基化胞嘧啶的碱基。纯化总DNA，并通过RT-PCR亚硫酸氢盐测序确定甲基化。该程序包括DNA纯化，亚硫酸氢盐处理和对照区域的RT-PCR扩增，该区域分为三个带有特定引物的子区域。比较经亚硫酸氢盐处理和不经亚硫酸氢盐处理获得的序列，以鉴定甲基化的胞嘧啶二核苷酸。此外，通过包括阴性对照来评估胞嘧啶从C到U的转化效率。有趣的是，主要在非C-磷酸盐-G（non-CpG）二核苷酸中以及在大多数含有调节元件的区域（例如OH或CSBI，CSBII和CSBIII）观察到mtDNA甲基化。这种新方法将促进有关来自具有不同病理或暴露于不同人群的毒性环境的患者样品中mtDNA甲基化模式的新信息的产生。

关键词: 线粒体表观遗传学，亚硫酸氢盐测序，甲基化，线粒体DNA，D环，RT-PCR。

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