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当代肿瘤药物靶点

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ISSN (Print): 1568-0096
ISSN (Online): 1873-5576

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Research Article

p62的过度表达通过促进ERK信号通路诱导自噬并促进鼻咽癌细胞的增殖，迁移和侵袭。

卷 20, 期 8, 2020

页: [624 - 637] 页: 14

弟呕挨: 10.2174/1568009620666200424145122

价格: $65

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摘要

背景：越来越多的证据表明，p62在肿瘤发生中起重要作用。 然而，关于p62与肿瘤侵袭和转移之间的相关性知之甚少。 另外，其在NPC（鼻咽癌，NPC）中的作用很少被研究。 目的：探讨p62对鼻咽癌发生，转移的影响。 方法：采用Western blotting，免疫荧光染色和免疫组化方法检测p62蛋白的表达。 随后，进行细胞活力，集落形成，迁移，侵袭和自噬测定。 通过ELISA检测血清中的抗p62自身抗体。 这些数据与临床病理参数相关。 结果：我们证实在鼻咽癌组织中p62明显上调。 此外，在NPC细胞系中，特别是在高度转移的5-8F细胞中，观察到了p62的高表达。 在体外，p62的下调抑制了5-8F细胞的增殖，克隆形成能力，自噬，迁移和侵袭，而p62的过表达在6-10B细胞中产生了相反的作用。 此外，我们证实p62通过激活ERK（细胞外信号调节激酶，ERK）促进NPC细胞增殖，迁移和侵袭。 临床分析表明，p62高表达与淋巴结转移和远处转移有关（P <0.05）。 鼻咽癌患者血清抗p62自身抗体升高，且水平与转移相关。 结论：我们的数据建立了以ERK为靶点的p62在NPC中的潜在决定因素，这为治疗NPC提供了新的途径。 此外，p62是潜在的生物标志物，其可能与NPC的肿瘤发生和转移密切相关。

关键词: 鼻咽癌，p62，转移，自噬，自身抗体，ERK，EMT。

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