Abstract
Background: 6-Gingerol (6G) and 6-Shogaol (6S) are the main active components of ginger. 6-Gingerol is known for its anti-metastatic and anti-invasive pharmacological activities on cancer cells, besides, 6-Shogaol also inhibits breast cancer cell invasion.
Objective: In this study, radioiodination (131I) of 6G and 6S was aimed. Additionally, it is aimed to monitor their incorporation behavior on breast cancer cell lines.
Methods: 6-Gingerol was isolated from the fresh ginger-roots extract, additionally, dehydrated to obtain 6-Shogaol. 6G and 6S were radioiodinated using iodogen method. Quality control studies of radioiodinated ginger compounds (6G and 6S) were performed by thin layer radio-chromatography. In vitro studies of radioiodinated ginger compounds on MCF-7 and MDA-MB-231 cells were performed with incorporation assays.
Results: 6-Gingerol and 6-Shogaol were radioiodinated (131I-6G and 131I-6S) in high yields over 95%. 131I-6S demonstrated higher incorporation values than 131I-6G on MDA-MB-231 cells. Incorporation behavior of 131I-6G and 131I-6S was similar to MCF-7 cells.
Conclusion: It has been observed that ginger compounds were radioiodinated successfully and 131I-6S have a noteworthy incorporation on MDA-MB-231 cells which is a known breast carcinoma cell line with highly invasive characteristics.
Keywords: 6-gingerol, 6-shogaol, radioidination, iodine-131, breast cancer cell lines, radio-chromatography.
[http://dx.doi.org/10.1016/j.foodchem.2014.02.086] [PMID: 24731320]
[http://dx.doi.org/10.1201/b10787-8]
[http://dx.doi.org/10.1158/1940-6207.CAPR-13-0420] [PMID: 24691500]
[http://dx.doi.org/10.1111/nyas.13375] [PMID: 28505392]
[http://dx.doi.org/10.1016/j.phanu.2017.01.001]
[http://dx.doi.org/10.1038/s41598-018-30522-6] [PMID: 30108263]
[http://dx.doi.org/10.1371/journal.pone.0126605]
[http://dx.doi.org/10.1007/s11418-019-01323-6] [PMID: 31177355]
[http://dx.doi.org/10.1016/j.taap.2018.11.001] [PMID: 30408433]
[http://dx.doi.org/10.1142/S0192415X15500767] [PMID: 26477795]
[http://dx.doi.org/10.1021/jf1029256] [PMID: 21090746]
[http://dx.doi.org/10.1016/j.fct.2006.11.002] [PMID: 17175086]
[http://dx.doi.org/10.1016/j.canlet.2013.04.014] [PMID: 23612072]
[http://dx.doi.org/10.1007/s10967-015-4082-9]
[http://dx.doi.org/10.1158/0008-5472.CAN-09-0491] [PMID: 19531649]
[http://dx.doi.org/10.1021/acs.jafc.5b00002] [PMID: 25686711]
[http://dx.doi.org/10.1186/1472-6882-7-44] [PMID: 18096028]
[http://dx.doi.org/10.1002/ptr.6134] [PMID: 30009484]
[http://dx.doi.org/10.1089/cbr.2017.2181] [PMID: 28358602]
[http://dx.doi.org/10.1016/S0969-8043(00)00337-7] [PMID: 11258523]
[http://dx.doi.org/10.1053/j.seminoncol.2010.06.014] [PMID: 20709209]
[http://dx.doi.org/10.1016/j.jnutbio.2007.05.008] [PMID: 17683926]
[http://dx.doi.org/10.1002/mnfr.200700157] [PMID: 18384088]
[http://dx.doi.org/10.1016/j.phytochem.2015.07.012] [PMID: 26228533]
[http://dx.doi.org/10.1590/1414-431x20154494] [PMID: 25923459]
[http://dx.doi.org/10.1016/B978-0-12-813768-0.00024-4]
[http://dx.doi.org/10.1016/j.semcancer.2015.09.007] [PMID: 26590477]
[http://dx.doi.org/10.1007/s00018-005-5017-3] [PMID: 15868411]
[http://dx.doi.org/10.1016/j.foodchem.2012.04.145] [PMID: 22868095]
[http://dx.doi.org/10.1002/jps.1116] [PMID: 11745724]
[http://dx.doi.org/10.13005/ojc/310156]
[http://dx.doi.org/10.1016/j.foodchem.2018.07.078] [PMID: 30174028]
[http://dx.doi.org/10.1371/journal.pone.0214893] [PMID: 31181065]
[http://dx.doi.org/10.1089/cbr.2014.1621] [PMID: 24983126]
[http://dx.doi.org/10.1002/med.20198] [PMID: 20099266]
[http://dx.doi.org/10.1016/j.yexcr.2018.02.018] [PMID: 29462602]
[http://dx.doi.org/10.1002/mnfr.200900125] [PMID: 20175081]