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Protein & Peptide Letters

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ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

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Mini-Review Article

A Comprehensive Guide to the Commercial Baculovirus Expression Vector Systems for Recombinant Protein Production

Author(s): Vibhor Mishra*

Volume 27, Issue 6, 2020

Page: [529 - 537] Pages: 9

DOI: 10.2174/0929866526666191112152646

Price: $65

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Abstract

The Baculovirus Expression Vector System (BEVS) is a workhorse for recombinant protein expression for over thirty-five years. Ever since it was first used to overexpress the human IFN-β protein, the system has been engineered and modified several times for quick and easy expression and scale-up of the recombinant proteins. Multiple gene assemblies performed on the baculovirus genome using synthetic biology methods lead to optimized overexpression of the multiprotein complexes. Nowadays, several commercially available BEVS platforms offer a variety of customizable features, and often it is confusing which one to choose for a novice user. This short review is intended to be a one-stop guide to the commercially available baculovirus technology for heterologous protein expression in the insect cells, which users can refer to choose from popular and desirable BEVS products or services.

Keywords: Baculovirus, insect cell, BEVS, protein expression, MultiBac, AcMNPV.

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Graphical Abstract

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