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Research Article

Pygo2的过表达增加人脐带间充质干细胞向心肌样细胞分化的能力。

卷 20, 期 4, 2020

页: [318 - 324] 页: 7

弟呕挨: 10.2174/1566524019666191017150416

价格: $65

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摘要

背景：我们以前的研究表明，果蝇中的Pygo（Pygopus）在成年心脏功能中起着至关重要的作用，而这在哺乳动物中可能是保守的。然而，其在人脐带间充质干细胞（hUC-MSC）分化为心肌细胞中的作用仍然未知。 目的：探讨pygo2在hUC-MSCs向心肌细胞分化中的作用。 方法：将第三代hUC-MSC分为两组：感染GV492-pygo2病毒的p +组和感染GV492病毒的p-组。感染和孵育3或21天后，进行定量实时PCR（qRT-PCR）以检测多能性标志物，包括OCT-4和SOX2。在感染后第7、14和21天分别通过免疫荧光检测Nkx2.5，Gata-4和cTnT。在转染病毒后一，二和三天，通过qRT-PCR分析了心脏相关基因的表达，包括Nkx2.5，Gata-4，TNNT2，MEF2c，ISL-1，FOXH1，KDR，αMHC和α-肌动蛋白。 3周。 结果：孵育三天后，与对照组相比，p +组hUC-MSC中多能干细胞标志物OCT-4和SOX2的表达没有明显变化（OCT-4：1.03±0.096 VS 1，P> 0.05，SOX2：1.071±0.189 VS 1，P> 0.05）;但是，在21天后，观察到显着下降（OCT-4：0.164±0.098 VS 1，P <0.01，SOX2：0.209±0.109 VS 1，P <0.001）。温育7天后，中胚层专门化标志物，如Nkx2.5，Gata-4，MEF2c和KDR的表达增加。孵育后第14天，p +组的心脏基因（例如Nkx2.5，Gata-4，TNNT2，MEF2c，ISL-1，FOXH1，KDR，αMHC和α-肌动蛋白）的表达相对于p −组（P，0.05）。综上所述，这些发现表明pygo2的过表达导致更多的hUCMSC逐渐分化成心肌样细胞。 结论：我们是第一个表明pygo2的过表达显着增强心脏基因（包括Nkx2.5和Gata-4）的表达，并促进hUC-MSC分化为心肌样细胞的方法。

关键词: 人脐带间充质干细胞，pygo2，过表达，分化，心肌细胞，Nkx2.5，SOX2。

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